Question: Design primer for a family of bacterial genes
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gravatar for David
3.1 years ago by
David160
David160 wrote:

Hello, I have a family of bacterial genes that are conserved. I have almost 3000 genes for that particuar family.

I would like to design pcr primers for sequencing amplicons for that particular family. On average the sequences are 600nt long and i want to find primer pairs for the miseq platform (roughly 2*250 = 500pb). Ideally the primers should amplify in between 440 and 500 nt.

What is the best strategy. ?

I have first created an alignment of the 3000 genes but don´t know how to move forward from there . I have used degeprime to create degenerated primers from the alignment.

Any iodea on how to move forward ?

pcr sequence sequencing • 1.1k views
ADD COMMENTlink modified 3.1 years ago by natasha.sernova3.7k • written 3.1 years ago by David160
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gravatar for natasha.sernova
3.1 years ago by
natasha.sernova3.7k
natasha.sernova3.7k wrote:

See my answer within this post.

C: Designing primers for family of genes

It also has a bacterial part.

ADD COMMENTlink written 3.1 years ago by natasha.sernova3.7k
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