Reciprocal best hit by blast
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7.5 years ago
qwzhang0601 ▴ 80

I am trying to get the mouse orthologs of my protein sequences from blind mole rate. Usually people consider "Reciprocal best hit" (which mean the matched pairs from each spices should be the best hit in two direction mapping). I just downloaded the standalone version of Blast, but I am not sure whether blastp can do this directly. Or I have to do the mapping in two directions and then find such "Reciprocal best hit" by myself.

Thanks

blast orthologs • 2.7k views
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