Hi everyone, I want to show the post-transcription interaction of MicoRNA with mature mRNA. I am looking for a server that can show this interaction and if not, then could anyone tell me about the interaction of MicroRNA with DNA? thanks for your time and attention
Are you interested in simply predicting which mRNAs a miRNA interact with (i.e. predicted targets of an miRNA)? Or are you looking to further determine whether there is a significant change in expression (including potential post-transcriptional regulation) of those targets?
Regardless, a good starting point might be to get lists of predicted target mRNAs for your miRNA. There are a number of tools available to do this, including: TargetScan: http://www.targetscan.org/vert_71/ PicTar: http://pictar.org miRanda: http://www.microrna.org/microrna/home.do
If you give me more details on exactly what you are interested in doing, I can try to help a bit more.
Hi,
Please search for miRNA in the "Integration with other data types" section at this paper.
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Dear Zahir, Hi,
Do you have any transcriptomic data of the species you are working with?
And maybe you are interested in some integrated analysis (e.g: 1 and 2) which is more complicated in non-model species.
~ Best
I dont have transcriptomic data but i know the microRNA and the gene sequences where it binds. The problem is, I could not find an online server that can be used to show the hybridization of microRNA with my gene mRNA. Could anybody suggest me any tool/sof or server plz?
Hi,
Please kindly put your comment and responds in the "add a reply" section, instead of "adding new answer". Thanks
Second, could I used the transcriptomic data where my gene is upregulated for microRNA targeting?