Question

Must I Have The .Sff Files In Order To Run Mira3

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13.7 years ago

Walter ▴ 150

I have only the fasta files and qual files and i need to run mira3. is it posibble to run mira3 and where can i obtain the .sff files.

mira • 2.6k views

ADD COMMENT • link updated 13.2 years ago by biobot 0.0.77.a.1099 6.2k • written 13.7 years ago by Walter ▴ 150

Ram · Answer 1 · 2010-08-19

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13.7 years ago

biobot 0.0.77.a.1099 6.2k

No, you do not require SFF files. If you have SFF files, the first step is to convert to Fasta, qual and traceinfo files. As you have Fasta and qual files, you should be able to skip this step. However, you may need a traceinfo file (or equivalent) to inform mira3 about parameters such as strain name and library insert sizes. The manual contains details of how to make a traceinfo file by hand, if you do not have one.

ADD COMMENT • link 13.7 years ago by biobot 0.0.77.a.1099 6.2k

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It specifically mentions on the feature page that it will accept FASTA and FASTQ input formats.

ADD REPLY • link updated 4.7 years ago by Ram 43k • written 13.7 years ago by User 59 13k