Question: after I use sva package to remove batch effect,can I use DESeq2 to make DE analysis?
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Ada • 0 wrote:
hello,here my question. If I use sva packages to remove the batch effect,which mean I need to normalize the read counts.But I want to make DE analysis with DESeq2 ,and that need raw read counts without normalization.and here the conflict is that I need to remove batch effect before differential expression analysis.How can I solve this problem ? I need your help .
