Entering edit mode
7.4 years ago
rplana6
•
0
Hi to everybody;
I am trying to execute a BIOPerl module Bio::Tools::Run::RemoteBlast but I get the error 403 Forbidden. I use a fasta file:
And the parameters I use are:
my $prog = 'blastn';
my $db = 'nt';
my $e_val= '1e-4';
my @params = ( '-prog' => $prog,
'-data' => $db,
'-expect' => $e_val,
'-readmethod' => 'SearchIO' );
my $factory = Bio::Tools::Run::RemoteBlast->new(@params1);
#change a query parameter
# This example shows how to change a CGI parameter:
$Bio::Tools::Run::RemoteBlast::HEADER{'MATRIX_NAME'} = 'BLOSUM50';
$Bio::Tools::Run::RemoteBlast::HEADER{'GAPCOSTS'} = '15 2';
# Si no ho posem dóna un warning
$Bio::Tools::Run::RemoteBlast::MODVERSION = 0;
#change a retrieval parameter
$Bio::Tools::Run::RemoteBlast::RETRIEVALHEADER{'DESCRIPTIONS'} = 1000;
#remove a parameter
delete $Bio::Tools::Run::RemoteBlast::HEADER{'FILTER'};
#$v is just to turn on and off the messages
my $messagesMngm = 1;
I always get the same error, no matter all changes in parameters I do.
Is there anybody who can help me please?
Thanks! Roberto
are you working behind a proxy ?
Hi again,
I've tried it at home and also at work. At home I have a normal Internet connection, at work there is a proxy, yes. Why? Is there a problem? I execute the BLAST via NCBI and it works correctly.
Thanks! Robert