Dear Friends, Hi (not native in Eng...)
I have used DIAMOND for creating a .daa file after blastx my transcriptome.fasta assembly against NCBI nr database with this script:
> diamond blastx -d nr -q '/home/Trinity_pathless.fasta' -o diamond-Trinity-daa -p 22 -f 100 --evalue 0.000001 --sensitive
Then I have imported it in the MEGAN6 community version (I have tried both approach (1) direct import and then create a MEGAN6 "RMA" file and (2) using Meganaizer tool - according to last lines of the MEGAN manual),
but the result has no taxonomic data!
Please help me in this regard and thank you in advance
NOTE: It seems that the MEGAN6 manual did not offer any guidance about blast taxonomy parameters.
NOTE2: If you are aware of any MEGAN6 problem-solver groups, please let me know.