Hello everyone, I'd like to explore the functional impact of some InDels on its protein product. So I annotated these mutations with Ensembl and got the corresponding transcript. Then, I translate these transcripts into peptides with InDels to do downstream analysis.
But some transcripts from the Ensembl with one or more "N" at the beginning of the sequence, so I don't know how to deal with these transcripts.
Bellow is a example transcript start with "N from Ensembl:
So, my questions are :
- why the transcripts with "N"?
- How to deal with "N" when we translate the transcript into peptides?
- Or should we just drop these transcripts with "N"?
Thank you very much.