I have a gene reference sequence I downloaded from NCBI: https://www.ncbi.nlm.nih.gov/gene/102267223#reference-sequences I've read a paper and wanted to do a little sanity check mostly for educational purposes. Basically I want to find primers in a reference sequence. The primers are for example:
E8-S GCTAACCTCTCTGGACCACCTTC E8-A TGTCAGCCTCAGGAACACCC
I could find Start primer, but couldn't find Abort primer. The command I am using to find primers is:
In : yak_2.seq.find("GCTAACCTCTCTGGACCACCTTC") Out: 35708
When I tried to search for reverse_compliment() did not find anything. Am I doing something stupidly wrong?