Entering edit mode
7.4 years ago
anasmalik324
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0
Good day everyone. i am working on a genome assembly and i have converted the SRA files into FASTQC with the help of SRA toolkit. Now i have to do the quality check and for tha i am using the FASTQC and it's always get stuck at 5% please help me out what could be the possible error that data size is 1.4GB. you can personally e-mail me on -censored-
What is the exact command you're running? Are you runnning fastqc with any flags? What is your machine's memory?
Using personal email addresses is discouraged, and for privacy reasons I removed your address.
Is the process using memory or is it completely dead/unresponsive? Try to have a look using
htoportop.You may have a corrupt fastq file. If you have the SRA# you can search EBI-ENA with that. You can directly download fastq files from there.