Convert Illumina 1.5 to Illumina 1.3

Question

[Abdul Rafay Khan]

I have fastq file in Illumina 1.5 encoding, i want to convert it into Illumina 1.3 encoding.

Answer 1

[Brian Bushnell]

If you want to convert the quality scores from ASCII-33 to ASCII-64, you can do so with the BBMap package like this:

reformat.sh in=x.fastq out=y.fastq qin=33 qout=64