Question: Problem with R package QuasR: qAlign function does not work
gravatar for cristian
3.9 years ago by
cristian240 wrote:

Dear all, I am trying to use QuasR to analyse my RNA seq data.

sampleFile <- "output/genome/ref/seq/samples_rna_single.txt"

genomeFile <- "output/genome/ref/seq/celegans.fa"

proj2 <- QuasR::qAlign(sampleFile, genome=genomeFile, splicedAlignment=TRUE, paired = 'no')

Error: output/genome/ref/seq/samples_rna_single.txt should contain the column names FileName1,FileName2,SampleName (paired end data)

I do not understand the error. I set the parameter 'paired' to 'no', so surely it should not except paired reads. Can anyone help, please? My samples_rna_single.txt file contains two columns named FileName and SampleName.

Thank you so much!


rnaseq qalign R quasr • 1.3k views
ADD COMMENTlink written 3.9 years ago by cristian240

there is no header in the sampleFile. that is it

ADD REPLYlink modified 3.0 years ago • written 3.0 years ago by ta_awwad280
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