Hello! I present my problem. I created with the command "makeblastdb" a database that contains my sequences of interest. Now I want to make a query: the file on which do the query (assume it's called NG_006672.fasta) contains a single sequence; I want to make a query on a substring of the sequence contained in my file, for example to take a substring of length 20. I would like to make a query being able to act on the indices of my query sequence
Please clarify what you mean by this? This request does not match the first part of your question.