Entering edit mode
7.3 years ago
lostisle
•
0
I'm using DESeq2 to analyze differential gene expression between two groups. As I understand, the log fold changes for genes with low counts (low mean expression) should be shrunken toward zero, but in the MA-plot (which I'm having trouble posting), they're shrunken toward -0.4 or so. Why is this so and how can I fix it? I'm worried all my LFC estimates are off. Thanks.
Also posted on Bioconductor: https://support.bioconductor.org/p/90380/