Hi guys, I have a protein around 200 Aa ( which structure is known). I have 3 different sequences of the same protein with one point mutation in each case. I will like to evaluated in a computational way the effects of these alterations. I try to model it using homology modeling online tools ( such as swissmodel) but the problem is that the resulting structures show no differences. This makes me think that maybe this method is not good enough to find the structural consequences of single Aa changes. I did also try de novo modeling tools ( QUARK), but the resulting structures are far away to be consider reals.
From similar posts I can see that there are many structural predictions tools (https://en.wikipedia.org/wiki/List_of_protein_structure_prediction_software) . But I was wondering if any of you guys have experience with any one that performs well for this kind of task.
Thanks for reading