stimulating 300 bp paired end reads for illumina data from ART software

Question

[dhwani.dholakia]

Is there any software to simulate 300 bp amplicon reads (illumina) using ART or is there any alternative software available for the same

[Medhat]

Grinder is a versatile open-source bioinformatic tool to create simulated omic shotgun and amplicon sequence libraries for all main sequencing platforms.

Grinder

also art_illumina

had the function of amplicon sequencing simulation

[dhwani.dholakia]

i have used art, however it limits to only 250 bp pair end reads. Is there any way to simulate 300 bp reads

[Medhat]

I think someone have issue like you can you have a look here

it is possible to simulate Illumina reads at longer lengths provided you generate the error profiles with ART using input files that have the desired length.

C: Art: Simulation Tools To Generate Synthetic Next-Generation Sequencing Reads.

[dhwani.dholakia]

Ya, i have read that before posting. However, sadly there was no solution on that post

Answer 1

[lakhujanivijay]

See this link https://omictools.com/read-simulators-category