Question: multiplex primers design software
0
gravatar for polys
4.2 years ago by
polys0
polys0 wrote:

Hi, I'm sort of new to multiplex pcr primers design, and I cannot seem to find a software that suits my needs....

I need to design 3 pairs of primers to detect 3 different microorganisms in one PCR reaction. I was wondering if there's any soft that can: - design 3 pairs of primers compatible with multiplex pcr - check that all of the primers are specific for their target microorganisms (this is, check against bacterial genomes)

Is there any software that can do this automatically for me?

Thanks in advance

multiplex pcr • 2.1k views
ADD COMMENTlink modified 4.2 years ago by WouterDeCoster45k • written 4.2 years ago by polys0

Designing Primers For Multiplex Pcr

ADD REPLYlink written 4.2 years ago by GenoMax96k

Hey, thanks for the reply. I've already tried PCRTiler, but it seems to work on only one microorganism at a time, since I cannot select more than one target in the Specificity section. Or am I missing something?

ADD REPLYlink written 4.2 years ago by polys0

I was just pointing out a past thread (have not used the software). Here is another option: MPprimes vs. PCRTiler for PCR Multiplexing

ADD REPLYlink written 4.2 years ago by GenoMax96k

thanks, I've already checked MPprimer as well, no luck either :-(

ADD REPLYlink written 4.2 years ago by polys0
0
gravatar for WouterDeCoster
4.2 years ago by
Belgium
WouterDeCoster45k wrote:

I'm not sure but I think this is something that might be what you need, or put you on the right track: http://primal.zibraproject.org/

ADD COMMENTlink written 4.2 years ago by WouterDeCoster45k

thanks! unfortunatedly this soft is not appropiate either. Thanks anyway!

ADD REPLYlink written 4.2 years ago by polys0
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