Entering edit mode
7.3 years ago
lrp.sailo
•
0
Hi all !! I have sent porcine PBMC samples for miRNA sequencing. After passing the quality check (RIN value = 6.9) I was informed that the sequencing was failed (number of reads were less ranging from thousands to 7 millions for 3 different samples) . Is there any possibility of failing sequencing after passing QC? What will be the reasons for getting less sequencing reads?
It sounds like you need to work with your sequencing facility and/or illumina tech support to understand what the problem is in this case. You may want to post this over at http://seqanswers.com.