Sorry if this is a rather basic question. I am trying to find a good solution on how to calculate fold change for qPCR data normalised to housekeepting genes across different conditions.
For example, I have this data: (CT value) Baseline condition Housekeeping gene: 30.7, 30.6, 30.6 Baseline condition Gene X: 33.0, 34.2, 33.5
Treatment condition Housekeping gene: 31.1, 31.4, 31.3 Treatment condition Gene X: 29.1, 29.4, 29.6
After I normalise the baseline condition gene X / Treatment condition gene X to the housekeeping gene by delta-delta CT, what is the correct formula to calculate log2 fold change of treatment over baseline condition please?