**20**wrote:

Hi,

I am trying to find deferentially expression microRNAs from my dataset which has four treatments. My experiment contains 4 treatments with 3 biological reps of each, and I have already normalized my data. Now I am looking to perform multiple comparisions on my select 200 miRNAs and obtain a p-value and adjusted p-value so as to determine deferentially expressed ones. 1. Which statistical test should I be using? - I am not sure if my data is normally distributed and/or has equal variance. 2. Packages like DESeq as for raw reads but that is not useful for me since raw reads has junk sRNA sequences that dilute my pvalue significantly. 3.IS there a simple way to perform this on excel/R/using a formula?

Thank you!