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7.3 years ago
fereshteh
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0
Hello all, The subject of my thesis is the evaluation of gene expression differences between two different species of mammals in a type of cancer. And six samples in each group of case and control are collected separately. At first I have two questions. What is the best protocol for extraction of RNA from PBMCs samples that are collected and stored at -70 for a while? To generate data, and studying the expression level, if it is better to sequence sample by individuals or by pooled samples? Please guide me with your experiences and suggestions
