after completing the de-novo assembly i got my transcriptome. is there any tool where i can upload that and find the gene expression?

Question

[smrithysimon]

after completing the de-novo assembly i got my transcriptome. is there any tool where i can upload that and find the gene expression? how i can get the pathway of new transcriptome using kegg?

Answer 1

[lakhujanivijay]

The ideal workflow would be :

From your assembled transcripts -> Run CD-HIT -> Run Transdecoder to get the Coding Regions Within Transcript -> blastx the CDS (functional annotation) / use the KEGG server