Entering edit mode
7.3 years ago
shiisgood
•
0
Hello,dear everyone. I want to download some RNA-seq raw data from NCBI by using Aspera. i used the command
~/.aspera/connect/bin/ascp -i ~/.aspera/connect/etc/asperaweb_id_dsa.openssh -k 0 -T -l200m anonftp@ftp-private.ncbi.nlm.nih.gov:/sra/sra-instant/reads/ByRun/sra/SRR/SRR285/SRR2854733/SRR2854733.sra .
and terminal told me that
ascp: Source file list not specified, exiting
i check the file in ncbi,the SRR2854733.sra in there . meanwhile, i try the SRR645370.sra
~/.aspera/connect/bin/ascp -i ~/.aspera/connect/etc/asperaweb_id_dsa.openssh -k 1 -T -l200m anonftp@ftp-private.ncbi.nlm.nih.gov:/sra/sra-instant/reads/ByRun/sra/SRR/SRR645/SRR645370/SRR645370.sra .SRR645370.sra
0% 6161KB 9.5Mb/s 3:13:
it is working. can everybody tell me the reason？do the aspera have limit on some sra? I'd appreciate any advice & suggestions.thank you very much.
