Question: Plasmid annotation explanation on Addgene
gravatar for piebeat09
4.1 years ago by
piebeat090 wrote:

I need some help trying to understand how annotations work on plasmids such as the one below. I do not understand the double layers structure, for example the light blue "ORF 2" has a annotation layer on top which is red for the GOI "firefly_Luciferase".

How can there be a ORF where a gene has already been inserted? Or does this mean that the gene can have a site-directed mutagenesis or be replaced by another GOI? Or is it just a double strand...

enter image description here

ADD COMMENTlink modified 4.0 years ago by Joe18k • written 4.1 years ago by piebeat090
gravatar for Joe
4.0 years ago by
United Kingdom
Joe18k wrote:

The blue is simply an annotation (probably old or curated by hand) which is showing you the ORF position. The red annotation gives you the actual CDS product. They are depicted side by side because they refer to exactly the same stretch of DNA.

In short, its a bad/redundant annotation.

ADD COMMENTlink written 4.0 years ago by Joe18k
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