Question: meta analysis of result of RNA-Seq data analysis
0
gravatar for hassan giahi
3.1 years ago by
IRAN
hassan giahi0 wrote:

hi all i have a problem for meta analysis of RNA-Seq defferential expression data that generated by CLC Genomic Workbench . in the result of RNA-Seq data analysis i see a total gene read column and tree p-value column that is the result of baggerly , edge and t-test . now , my question is that Which of them (3 type of p-value ) should be used for meta-analysis ??? is it impossible that i used the total gene read for meta analysis??

thanks giahi

ADD COMMENTlink modified 3.0 years ago by Biostar ♦♦ 20 • written 3.1 years ago by hassan giahi0

Could you explain more about your experimental setup? Essentially, of what are you doing a meta-analysis?

ADD REPLYlink written 3.1 years ago by WouterDeCoster42k

thank you for your answer my porpose in meta analysis is that : the application of one treatment material on breast cacer cell line is effective or not ??? and for this reason i want to do a meta analysis ...but i do not know that witch column of rna seq result data ( that analysed by CLC ) should be used for meta analysis ??? my rna seq output have this column : gene id , expression value .... , total gene read , p-value , .... i think that 3 method for calculate the p-value is : 1- baggerly 2- edge 3 t-test ??? now , for meta analysis , whitch of this column /method can i used ؟؟؟ thank you so much giahi

ADD REPLYlink written 3.1 years ago by hassan giahi0
2

Meta-analysis can mean everything. Do you want just to see how many differentially expressed genes are there, do you want to see if there is any significant difference between the samples in general, or do you want to see if the differential expression you see is reliable?

ADD REPLYlink written 3.1 years ago by Macspider3.0k
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