ChIP Real Time PCR analysis
I have done a ChIP-PCR (ChIP with H3K4me3 antibody). I just want to ask how to analyse ChIP PCR data. I am putting my positive and negative control Ct values. Positive Control IP Ct= 27.746 and Input Ct=23.956, Negative Control IP Ct=30.4935 and Input Ct=26.082. I am calculating ddCt using dCt(negative control) =IPCt- InputCt which comes=30.4935-26.082=4.4115
dCt(positive control) =IPCt- InputCt which comes= 27.746-23.956=3.79
ddCt= dCt(negative control)-dCt(positive control)= 4.415-3.79=0.3862
And putting ddCt value on the power of 2 it comes 1.3
The fold enrichment is 1.3? Am I correct? And how to draw the graph?
Topics
How to analyse ChIP PCR data? - ResearchGate. Available from: https://www.researchgate.net/post/how_to_analyse_ChIP_PCR_data [accessed Jan 10, 2017].
