NGS RNA-seq data for gene expression

Question

[Light]

Do I need to take control and disease sample SRA data for studying viral-host interaction and gene expression of non-model virus? if yes how much of the both samples are to be considered to study. I am new to NGS and research, so please guide me.

[Medhat]

Are you asking about biological replicate? as I see you are using SRA so actually you are controlled by the experiment design of the one who published the data.

So in general if you are asking about biologcal replicate you need at least 3 and yes you need both (healthy and diseased )to compare the effect between healthy and diseased one

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Answer 1

[Devon Ryan]

1. Yes, you'll need both types of samples.
2. More is always better. Try to avoid creating a batch effect, of course.

Answer 2

[Light]

Thank you for quick response