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• Software to analyse mutations in sanger sequencing files
  I am interested in finding a free software package to analyse sanger sequencing files (ab1). The...
• Phangorn bootstrap option
  I am using phangorn, maximum likelihood to estimate tree. optim.pml process, I used rearrangemen...
• Known CRISPR KO effects on gene expression
  I am looking for genes whose knockout with CRISPR/Cas9 (Indels) has known, strong effects on the ...
• CRISPR-Cas9 genome-scale screen
  When we do a CRISPR-Cas9 genome-scale screen, how can we make a relationship between genotype and...
• Looking CRISPR-Cas9 sequencing Data
  Hello, for my project I'm analyzing the mutational outcomes of CRISPR-Cas9 cleavage. Currently, ...
• plot the number of variations within a certain window
  we have used `crispr-cas9` technology which was followed by whole genome sequencing. I have got t...
• Getting the coordinates of targeted locations by sgRNAs
  I have performed `CRISPR-CAS9` exprimrnt which was followed by `WGS`. now I am trying to find the...
• CRISPResso analysis software
  Hi, I got the sequencing data of Miseq, and tried to use CRISPResso to do the analysis since I am...
• Is there any software can simulate RNA:DNA binding stability? Thanks!
  There exist some mismatches. I need to get some stabilities value. I want to apply it into CRISPR...
• Crispr-cas9 screen analysis mageck
  Hi friends! Now I have a batch of paired-end sequencing data，I don't know whether mageck can hand...
• CRISPR/Cas9 screen analysis: reads R1 and R2 mixed
  Hi! I want to analyse data from a CRISPR/Cas9 screen (control vs. treatment) and I'm using Mageck...
• INDELs frequency at the on-target site in CRISPR experiment
  I have WGS data from a CRISPR experiment. my goal is to get the frequency of INDELs in the on-tar...
• How to find which residues in a protein which are targetable by CRISPR
  Hello, I have some protein X with a known protein sequence which has both its cDNA and genomic s...
• Pooling WT and CRISPR/Cas9 KOs cells in the same scRNAseq sample and then demultiplex by puromycin resistance cassette expression
  Library preparation for single cells RNA sequencing ends being the most expensive part of the of ...
• Find The Second Best Maximum Likelihood Tree
  Hi All Generally the phylogentic program gives the best Maximum likelihood tree. Do any one know...
• Haplotype Frequencies And Maximum Likelihood Estimation / Expectation Maximization Algorithm
  Does anyone have a numerical example on how the EM algorithm can be used to determine haplotype f...