What 2 indicates in 2*150 bp, 2*300 bp maximum read length from Illumina website http://www.illumina.com/systems/sequencing-platforms.html?

Question

[saranpons3]

Dear Bioinformaticians,

I was looking at various Illumina sequencing platforms here http://www.illumina.com/systems/sequencing-platforms.html.

What 2 indicates in 2x150 bp, 2x300 bp maximum read length from Illumina website http://www.illumina.com/systems/sequencing-platforms.html?

I would be grateful if anybody can answer this?

[WouterDeCoster]

I edited your post because you use (probably not desired) markup, e.g. * will result in text in italics

Answer 1

[WouterDeCoster]

That refers to the direction(s) of sequencing: paired end sequencing. Essentially, in 2x150bp you are sequencing once 150bp in one direction of the molecule, and once in the other direction.

If you have a molecule of 400bp you would sequence 150bp from each side, leaving a gap of 100bp in the middle.

[saranpons3]

Hello WouterDeCoster,

Thanks for editing my question and the answer. If I assemble forward and reverse end reads where each of them are of length 150 bp , then saying "assembling the reads of 150 bp is correct" or saying "assembling the reads of 300 bp is correct" ?

Also, I'm looking for the Denovo assemblers which can assemble reads of length 300 bp. Do you know any assemblers which can assemble reads of 300 bp?

[WouterDeCoster]

No, you don't have reads of 300bp then. You have paired end 150bp reads.

For a bunch of tools for assembly, have a look at omictools. I can't give you more accurate advice for this, perhaps someone else.

[saranpons3]

Hello WouterDeCoster, Thanks for answering my question. As you said I'll look at omictools website.