I have 16 data for which I have generated alpha diversity data for using the estimate_richness function in phyloseq.
Group1: Observed Chao1 se.chao1 ACE se.ACE Shannon Simpson InvSimpson Fisher estimateR.OTU. 815 986.3829787 33.71773796 974.0515577 15.60638486 3.786528741 0.958708047 24.21779397 89.82207284
Group2: Observed Chao1 se.chao1 ACE se.ACE Shannon Simpson InvSimpson Fisher estimateR.OTU. 767 956.4186047 37.40434526 938.6584631 15.36714985 3.49141784 0.936417811 15.72767487 82.34122795
I have grouped the data rather than produce individual diversity estimates and then what I want to do is see if there are any statistically significant differences between these values for specific groups, although I'm not quite sure how to do this?
Would it be better to generate diversity data for each sample in the group and then use these in a T-test? Or if I have more groups, an ANOVA?