Question: Meta-analysis of RNA-seq Data
gravatar for pixie@bioinfo
3.2 years ago by
pixie@bioinfo1.4k wrote:


I have rna-seq data from two different tissues and different stress conditions. I have performed adapter removal using Cutadapt and I have used TopHat for alignment to the reference genome. What is the ideal method to perform differential gene expression analysis? Should I perform CuffDiff on individual stress-tissue dataset, extract the FPKM files from each of the experiments and merge them using some normalization method ? I need a way to normalize the read-counts across all the stress-tissue datasets because I want to construct co-expression network using RNA-seq data. Any suggestion will be very helpful. Thanks.

rna-seq • 1.5k views
ADD COMMENTlink modified 3.1 years ago by Biostar ♦♦ 20 • written 3.2 years ago by pixie@bioinfo1.4k

If you have no biological replicates, your dataset doesn't actually tell you very much.

ADD REPLYlink written 3.1 years ago by swbarnes27.5k
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