reads distribution from the reference

Question

[Alex]

Hi everyone , I want to classify my sequenced reads into introns,exons,,3-UTR,5-UTR,while have no ideas how to Separate it.dose anyone have any ideas? Thanks

Thanks

Alex

[GenoMax]

If you have a reference genome available then you align your reads to that reference and then can use a GTF (a gene annotation format file) to assign the mapped reads to their class.

[Alex]

Thanks ,I will try it