Question: Get normalized count Data using DESeq2 in R
0
gravatar for Krish Srinivasan
2.4 years ago by
Canada
Krish Srinivasan10 wrote:

Hello everyone,

I have 54 HT-seq Count files from an old experiment that I am reanalyzing. They are composed of combinations of the 2 treatment groups (Wildtype and Treatment), 3 exposures (water, tBOOH, diquat), and 3 times points. I need to create a matrix of the normalized count data for each gene in each sample. I am not sure how to start, any help would be appreciated.

Thanks,

rna-seq R • 5.2k views
ADD COMMENTlink modified 2.4 years ago by Sentinel156120 • written 2.4 years ago by Krish Srinivasan10
2
gravatar for WouterDeCoster
2.4 years ago by
Belgium
WouterDeCoster39k wrote:

What you are looking for is extraction of counts after variance stabilizing transformation (vsd/vst) or rlog normalization. You can find both described clearly in the DESeq2 manual (2.1.2 Extracting transformed values).

ADD COMMENTlink modified 2.4 years ago • written 2.4 years ago by WouterDeCoster39k
1
gravatar for Sentinel156
2.4 years ago by
Sentinel156120
Melbourne, Australia
Sentinel156120 wrote:

Hi, this is pretty simple using DESeq2's counts function and has been asked many times before. See here and here for examples.

ADD COMMENTlink written 2.4 years ago by Sentinel156120
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