I got a fastq files from core and they loaded same library on two different lanes. I read the posts of people that deal with this problem and they recommend to merge these file after converting to bam. My question is next, why I cannot merge these files using tophat into accepted_hits.bam by using these files like technical replicates? What will be the difference between these two methods. I plan to use cufflinks for the analysis of the DE. Thank you for the help.
Thank you very much for help.