Question: Do the BLAST and BLAST+ algorithms ignore the NNs in scaffolds/genomes or not?
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gravatar for mirza
2.2 years ago by
mirza80
India
mirza80 wrote:

Hi, I am new to NGS/ genome assembly field. I am given data and assembly (done by someone else) to evaluate if it is correct and if not, do the assembly myself. To check the given draft assembly, I mapped the largest scaffold of the existing reference genome (about 4Mb), using it as query to our draft assembly with blast+ and found that the query scaffold maps to 195 different scaffolds of the draft assembly. 1. I think this means that the assembly is highly fragmented?

The person is justifying this by saying that it is due to the presence of several gaps (Ns) in the query scaffold. Can someone please shed some light on this matter?

blast algorithm • 702 views
ADD COMMENTlink modified 2.2 years ago • written 2.2 years ago by mirza80

Not answering your question, but instead recommending QUAST for assembly quality evaluation.

ADD REPLYlink written 2.2 years ago by 5heikki8.3k

Thanks but I know about Quast and have all the intentions to use it but this is something very basic which generally doesn't come to mind. So, I want to clarify and what better platform than Biostar.

ADD REPLYlink written 2.2 years ago by mirza80

Please use ADD REPLY/ADD COMMENT to respond to existing posts to keep threads logically organized.

ADD REPLYlink written 2.2 years ago by genomax63k

I apologize. I realized after sending it.

ADD REPLYlink written 2.2 years ago by mirza80

No need to apologize. Just wanted to make you aware in case you did not know.

ADD REPLYlink written 2.2 years ago by genomax63k
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