The database for GRCH 37 for rBLAST
0
0
Entering edit mode
7.3 years ago
ylaguka • 0

Hello, I am trying to use blast in R. It requires downloading the database to a local file. I'm having trouble finding the database for human genome grch37 (I will settle for grch38 too). I've tried refseq_genomic.242 and human_genomic_transcript, none of it returned the right result.

I've read the Manual for the database but couldn't find more detailed explanations on the exact database I'm looking for. The source where I was looking is ftp://ftp.ncbi.nlm.nih.gov/blast/db

Been stuck on it for a while, any help is appreciated, thanks!

blast R • 1.9k views
ADD COMMENT
1
Entering edit mode

You could get the hg19 genome sequence from UCSC here. Uncompress and then cat individual chromosome files into a single large file. Create blast indexes using makeblastdb and then use those indexes for your program.

ADD REPLY
0
Entering edit mode

Thank you for the suggestion. I was thinking about doing it that way. As it seems difficult to locate the the exact grch37 database the blast website is using, I will use the makeblastdb. Thanks again.

ADD REPLY

Login before adding your answer.

Traffic: 2870 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6