Question: Perl::ForkManager does not speed up ATCG calculation
gravatar for BioGeek
13 months ago by
BioGeek100 wrote:

I am new to parallel programming, and today I decided to test the Perl ForkManager module.

I am reading a multifasta infile and calculating ATCG percentage for each sequence. I tried to fork it to 5 different thread to speed up. Unfortunately, it takes more time with ForkManager than normal. What am I doing wrong in following code?

use strict;
use Parallel::ForkManager;
use Bio::SeqIO;

#usage: perl <multi fasta infile>

my %sequences;
my $seqio = Bio::SeqIO->new(-file => "$ARGV[0]", -format => "fasta");
while(my$seqobj = $seqio->next_seq) {
    my $id  = $seqobj->display_id;    # there's your key
    my $seq = $seqobj->seq;           # and there's your value
    $sequences{$id} = $seq;

  my $max_procs = 5;
  my @names = keys %sequences;

  # hash to resolve PID's back to child specific information
  my $pm =  new Parallel::ForkManager($max_procs);

 # Setup a callback for when a child finishes up so we can
  # get it's exit code
  $pm->run_on_finish (
    sub { my ($pid, $exit_code, $ident) = @_;
      #print "** $ident just got out of the pool ".
        "with PID $pid and exit code: $exit_code\n";

    sub { my ($pid,$ident)=@_;
     #print "** $ident started, pid: $pid\n";

    sub {
      #print "** Have to wait for one children ...\n"

  foreach my $child ( 0 .. $#names ) {
    my $pid = $pm->start($names[$child]) and next NAMES;
    $pm->finish($child); # pass an exit code to finish

  print "Waiting for Children...\n";
  print "Everybody is out of the pool!\n";

sub checkATCG {
my $name=shift;
my $DNA=$sequences{$name};
my $length=length $DNA;
my $a=($DNA=~tr/A//);
my $b=($DNA=~tr/C//);
my $c=($DNA=~tr/G//);
my $d=($DNA=~tr/T//);
my $Total=$a+$b+$c+$d;
my $GC=($DNA=~s/GC/GC/g);
my $AT=($DNA=~s/AT/AT/g);
my $GCper=($GC/($Total)*100);

forkmanager parallel speed perl • 519 views
ADD COMMENTlink modified 13 months ago by Ram13k • written 13 months ago by BioGeek100

The way that you calculate the percentages is not efficient in check ACGT, using repeated regexes over the entire sequence simply to count number of characters, where it could simply loop through the string to count characters or similar. I can't comment on how the ForkManager works but that to me is a more obvious place to optimize.

ADD REPLYlink written 13 months ago by cmdcolin700

note: I didn't mean to sound so negative and offtopic in this reply as I know the forkmanager was really the topic in question :)

ADD REPLYlink written 13 months ago by cmdcolin700
  • Perl::ForkManager is useful when you're actually computing something that takes time, longer than time(checkATCG) [1]
  • checkATCG needs some work


ADD REPLYlink modified 13 months ago • written 13 months ago by Cornel30

If you are interested in exploring parallel programming, I suggest you look into Go or Java! They make it very easy to do efficiently.

ADD REPLYlink written 13 months ago by Brian Bushnell15k
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