Question: How is an RNA template to be reverse transcribed into cDNA chosen?
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gravatar for und1agn0s3d
2.2 years ago by
und1agn0s3d0 wrote:

I read the paper, "Bioluminometric Method for Real-Time Detection of Reverse Transcriptase Activity", and was wondering how they chose the E2PN template oligonucleotide? I'm assuming it was partially chosen to not match any known genetic sequences. I'm not sure of the design process in general though?

E2PN (5′-CGACGATCTGAGGTCATAGCTGTTTCCTGTGTGAACTGGCCGTCGTTTTACAACG-3′)

reverse transcriptase • 475 views
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