Question: Structural Variation Discovery: Delly's Output
1
gravatar for novice
3.2 years ago by
novice950
United States
novice950 wrote:

I have a question about Delly's MAPQ value. Before calling SVs on my data, I filtered all reads with MAPQ 20 or less. In Delly's output, however, many of the SV predictions have MAPQ values less than 20. So my question is how does Delly calculate MAPQ score?

Thanks.

delly mapq sv • 1.4k views
ADD COMMENTlink modified 2.7 years ago by trausch1.4k • written 3.2 years ago by novice950
0
gravatar for trausch
2.7 years ago by
trausch1.4k
Germany
trausch1.4k wrote:

For SV discovery, delly uses MAPQ and filters reads with MAPQ < q where q is by default 1.

For SV genotyping, delly hashes the mapping qualities of both pairs and uses the minimum of these 2 mapping qualities as the paired-end quality. For bwa alignments it uses the AS tag (alignment score) instead of MAPQ because that worked better in genotyping benchmarks.

ADD COMMENTlink written 2.7 years ago by trausch1.4k
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