How can I select my Chip-seq genes (targets) in my Rna-seq data?
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4.7 years ago
LuisNagano ▴ 90

Hi, I need some help! I want to select my target genes generated by my ChIP-seq (HOMER - genes symbols) within my list of differentially expressed genes from RNA-seq (Cuffdiff output), so I can identify my target genes ids, “XLOC_” gene ids generated by cufflinks, (like select a complete line from my cuffdiff file where I find my target gene), this way I can plot the heatmap of these genes using RNA-seq data. How can I do this in a simple way?

->Annotated genes (HOMER output)

  • SULT1B1
  • LHFPL
  • ZMYND8
  • RAD23B ...

->Cuffdiff table file (RNA-seq)

  • gene_id gene symbol locus
  • XLOC_000001 DDX11L1 chr1:11868-31109
  • XLOC_000002 MIR1302-2 chr1:11868-31109
  • XLOC_000003 OR4G4P chr1:52472-53312 ...
RNA-Seq ChIP-Seq Cufflinks Cuffdiff • 1.7k views
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Hello LuisNagano!

It appears that your post has been cross-posted to another site: http://seqanswers.com/forums/showthread.php?t=74116

This is typically not recommended as it runs the risk of annoying people in both communities.

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What do you mean with "in a simple way"? Does it mean that you know how to use R for instance?

With R you can do this simple namely, your Homer output seem to be gene symbols, which I recognize in the second column of your cuffdiff table.

So import the files in R and use for example: %in%

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4.7 years ago

It looks like your target genes in ChIP-seq (HOMER output) and gene symbols in Cuffdiff table are in HGNC notation http://www.genenames.org/ So they should match exactly.

In order to select all lines from cuffdiff output that have gene names from the list of HOMER output in the second column you can run:

awk 'NR==FNR{HOMER_LIST[$1]=$1}(NR!=FNR&&HOMER_LIST[$2]){print $0}' homer_output_file cuffdiff_output_file

NR==FNR is true when awk reads the first file. That way all gene names from the first file will be stored in memory as an array HOMER_LIST. NR!=FNR is true when awk read the second file. Using && it also tests if second column element $2 from each row of the second file can be found in HOMER_LIST. As a result awk prints to stdout the complete line $0 from cuffdiff file where it can find gene name from that line in the target gene file.

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Thanks Petr, works very well!

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If this answer was helpful it is appropriate to upvote it, and if this answer resolved your question completely you can 'accept' the answer, as such marking your question as solved.

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how do I mark it as solved?

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It seems you (or someone else) already did that. Marking as solved is done by accepting the answer.

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