Dear Biostars, Hi.
I have used DESeq2 for my RNA-seq DEG analysis (de novo assembly using Trinity, RSEM, DESeq2) I have used FDR=0.05 as my threshold.
Now I have some genes that in similar researches was up-regulated in one condition (e.g in males) but in my results the same gene has the log2FC = 5 AND the FDR is 0.1 or more.
What is the best decision for me now ?
I appreciate any helpful guidance