Entering edit mode
7.2 years ago
L. A. Liggett
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120
In the past I have PCR amplified my cDNA for RNASeq. Is this necessary in all cases to do? I'm wondering if it is possible to just purify out a large enough amount of RNA, make cDNA from this and directly sequence.
Certainly. There are protocols for PCR-free RNAseq even from a single cell, an example.
Perfect, this is exactly what I was looking for.
Not exactly a bioinformatics question, but I see you already received the answer you needed.
you might refer also to the review by william greenleaf describing applications of next generation sequencing to analysis of epigenetics. while RNA seq is not the main focus of the paper, there is good background in this review that will enable you to get perspective on the above question.