Question: PCR Amplification necessity in RNASeq
3.9 years ago by
L. A. Liggett • 120
Broad Institute, Harvard Medical School, Boston Children's
L. A. Liggett • 120 wrote:
In the past I have PCR amplified my cDNA for RNASeq. Is this necessary in all cases to do? I'm wondering if it is possible to just purify out a large enough amount of RNA, make cDNA from this and directly sequence.
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