Question: PCR Amplification necessity in RNASeq
0
gravatar for L. A. Liggett
3.9 years ago by
L. A. Liggett120
Broad Institute, Harvard Medical School, Boston Children's
L. A. Liggett120 wrote:

In the past I have PCR amplified my cDNA for RNASeq. Is this necessary in all cases to do? I'm wondering if it is possible to just purify out a large enough amount of RNA, make cDNA from this and directly sequence.

rna-seq • 1.4k views
ADD COMMENTlink written 3.9 years ago by L. A. Liggett120
2

Certainly. There are protocols for PCR-free RNAseq even from a single cell, an example.

ADD REPLYlink modified 3.9 years ago • written 3.9 years ago by GenoMax94k

Perfect, this is exactly what I was looking for.

ADD REPLYlink written 3.9 years ago by L. A. Liggett120

Not exactly a bioinformatics question, but I see you already received the answer you needed.

ADD REPLYlink written 3.9 years ago by WouterDeCoster45k

you might refer also to the review by william greenleaf describing applications of next generation sequencing to analysis of epigenetics. while RNA seq is not the main focus of the paper, there is good background in this review that will enable you to get perspective on the above question.

ADD REPLYlink written 3.9 years ago by Vincent Laufer1.1k
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