Question: Making fasta files from gtf and reference genome
gravatar for Olajucan
3.5 years ago by
Olajucan0 wrote:

Hi, I'm working on RNAseq data analysis.

I used HISAT2 and Stringtie to make reference transcriptome and gtf files.

Since my material is wheat, whose reference genome was released a month ago but annotation isn't completed yet,

I have to extract sequences of transcripts as fasta format to annotate them.

For that purpose, Is GFF utilities working on that?

Or, have any good ideas for annotating transcripts?

rna-seq • 1.5k views
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