Question: Why Ribo-seq not as popular as RNA-seq?
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gravatar for vasanth.m.29
2.1 years ago by
vasanth.m.290 wrote:

Hi all,

I would like to know the difference between the Ribo-seq and RNA-seq and why Ribo-seq is not as popular as RNA-seq. I know that in both the profiling techniques we sequence the mRNA, but in Ribo-seq we sequecne the only cDNA that is actively translated.

rna-seq ribo-seq • 1.0k views
ADD COMMENTlink modified 2.1 years ago by Devon Ryan88k • written 2.1 years ago by vasanth.m.290
1
gravatar for Devon Ryan
2.1 years ago by
Devon Ryan88k
Freiburg, Germany
Devon Ryan88k wrote:

Ribo-seq is a much more complicated experiment. Further, you won't get any non-coding species in Ribo-seq. RNAseq is cheap and easy, though I wouldn't be surprised if Ribo-seq becomes a bit more popular over time.

ADD COMMENTlink written 2.1 years ago by Devon Ryan88k

Thank you Mr. Ryan, But, If we want to do a gene expression analysis wouldn't it be more sensible to do a Ribo-seq rather than a RNA-seq.

ADD REPLYlink written 2.1 years ago by vasanth.m.290

If you're willing to put in the extra effort then sure.

ADD REPLYlink written 2.1 years ago by Devon Ryan88k
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