Hi, I'd like to create a bed file defining subsequence (randomly taken 100-400bp, different for each input sequence) of 1kb sequences in bed file. I've looked of course at bedtools, and searched the web but haven't find anything useful.
bedtools random will generate fixed length sequences restricted by the chromosomes' boundaries
As I'd like to use bed file to extract sequences from genome I've also considered extraction of 1kb fasta sequences (using my bed file) and trimming it. But haven't find the answer how to do that pseudo-random trimming.