Hierachical cluster analysis using arraytrack select auto scale data?

Question

[tud55122]

I'm doing a hierarchical cluster analysis using arraytrack and I use a RNA-seq Z-score table. In my case, should I select auto scale data or not? I'm a bit confused. I tried both and the clusters look so different. Since the Z-score table is already normalized, I assume that re-normalization is not necessary.

Thanks, Hang

[shunyip]

I agree. Z-scores are normalized and have mean of zero and standard deviation of one. So, I believe further scaling should not be necessary.