**90**wrote:

I am working on RRBS data. Someone suggested me to use M-values for the calculation of differential methylation. In my understanding beta-values/m-values are used to measure methylation level in array based method. In sequencing data we get single base pair resolution methylation level after methylation call. Hence, no need for calculating beta-values/m-values.

I would like to make sure that I am right. Could you please confirm that beta-value/m-value calculation is not needed for sequencing data?

**97k**• written 3.7 years ago by Tori •

**90**