Does anyone know why when doing a de novo assembly of a bacterial isolate and then mapping back the same reads to the contigs, there are times when there is one or two nucleotides(out of 5 million) that mapping will call it completely opposite of the contig, for example the contig will say at position 25 there is G and the pileup file from mapping will say that at position 25 all the reads are a C. I assuming its an issue with repeat regions but don't have any evidence to tell me thats the reason why. I have seen it using velvet and spades as the de novo assemblers and bowtie2 and bwa as the mappers.
Question: denovo assembly and mapping
3.0 years ago by
andrewdavis3 • 0
andrewdavis3 • 0 wrote:
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