Entering edit mode
7.1 years ago
katherine.lavoie
•
0
Hello!
I run this line:
VelvetOptimiser.pl -d 'outputfolder' -s 21 -e 91 -f '-fasta -shortPaired ./myfile.fasta'
and velvetoptimizer always gives me a result of 31 as my ideal Kmer. it doesnt even test beyond that.
I dont really understand what I am doing wrong can anyone help me or explain my VelvetOptimizer always goes to its default parameter for kmer length to test?
Thank you!
By default velvet uses a maxkerlength of 31 which is what you are seeing. You need to re-compile
velvet
if you want to use longerkmers
. Do you have a reason to believe velvet could benefit from using longer k-mers?I will link these threads on how to make the necessary change: Velvet can't handle k-mers as long as X! We'll stick to 32 if you don't mind. and Question: Can't change MAXKMERLENGTH on Velvet