I run this line:
VelvetOptimiser.pl -d 'outputfolder' -s 21 -e 91 -f '-fasta -shortPaired ./myfile.fasta'
and velvetoptimizer always gives me a result of 31 as my ideal Kmer. it doesnt even test beyond that.
I dont really understand what I am doing wrong can anyone help me or explain my VelvetOptimizer always goes to its default parameter for kmer length to test?