Question: Normalization read counts scRNA seq with spike ins
0
gravatar for tetapunto
2.3 years ago by
tetapunto0
tetapunto0 wrote:

Hi,

I'm working with data obtained with sc-RNA seq where we used ERCC spike In to control for transcriptional amplification. I have some troubles with read counts normalization.

My question is: Is it possible to normalize read counts from a cell with the sum of spike ins read counts in that cell?

sequencing rna-seq • 958 views
ADD COMMENTlink written 2.3 years ago by tetapunto0
1

Your question sounds similar to SAMstrt. Maybe you can take a look.

ADD REPLYlink modified 2.3 years ago • written 2.3 years ago by shunyip180
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1252 users visited in the last hour